Chimeric glycoproteins and pseudotyped lentiviral vectors
US9090908B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 14, 2012 |
| Grant date | Jul 28, 2015 |
| Priority date | — |
| Expiry date | Nov 14, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/50
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method is provided for producing a recombinant pseudotyped viral vector particle wherein a cell is transfecting with (i) at least one vector construct; (ii) at least one packaging construct; and (iii) an expression construct encoding a chimeric glycoprotein encoded by a nucleic acid comprising the sequence of SEQ ID NO: 1 to yield a producer cell, followed by culturing the producer cell in a medium; and separating the producer cell from the medium to recover the recombinant viral vector particle from the medium. Vectors obtained in this manner have significantly higher titers than vectors coated with the parental non-chimeric glycoprotein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.