Patent · US Active

Method for purifying recombinant FSH

US9096683B2 · kind B2 · utility

0Cited by

5References

26Claims

0Family size

Assignee

ratiopharm GmbH · DE

Inventors

Christian Scheckermann · Ehrenkirchen, DE
Dietmar Eichinger · Schwetzingen, DE
Stefan Arnold · Munster, DE

Key dates

Filing date	Apr 1, 2010
Grant date	Aug 4, 2015
Priority date	—
Expiry date	Jul 22, 2030

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61K38/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

The present invention relates to a method for purifying a recombinant follicle stimulating hormone (FSH) or recombinant FSH variant. The method comprises the steps of subjecting a liquid containing a recombinant FSH or recombinant FSH variant to an anion exchange chromatography, to a hydrophobic interaction chromatography, and to a dye affinity chromatography, wherein these chromatographies may be performed in any order, and wherein the method neither comprises a weak anion exchange chromatography nor a reverse phase chromatography. The method of purification results in a high yield of recombinant FSH having a desired degree of purity. The obtained FSH is especially useful for the prophylaxis and treatment of disorders and medical indications where FSH preparations are considered as useful remedies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.