Microfluidic device for acoustic cell lysis

US9096823B1 · kind B1 · utility

Assignee

• Sandia Corporation · US

Inventors

• Darren W. Branch · Albuquerque, US
• Erika Jane Cooley · Albuquerque, US
• Gennifer Tanabe Smith · Albuquerque, US
• Conrad D. James · Albuquerque, US
• Jaime L. McClain · Albuquerque, US

Key dates

• Filing date: Aug 31, 2010
• Grant date: Aug 4, 2015
• Expiry date: May 1, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00554
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microfluidic acoustic-based cell lysing device that can be integrated with on-chip nucleic acid extraction. Using a bulk acoustic wave (BAW) transducer array, acoustic waves can be coupled into microfluidic cartridges resulting in the lysis of cells contained therein by localized acoustic pressure. Cellular materials can then be extracted from the lysed cells. For example, nucleic acids can be extracted from the lysate using silica-based sol-gel filled microchannels, nucleic acid binding magnetic beads, or Nafion-coated electrodes. Integration of cell lysis and nucleic acid extraction on-chip enables a small, portable system that allows for rapid analysis in the field.