7 α-hydroxysteroid dehydrogenase knockout mutants and use therefor

US9096825B2 · kind B2 · utility

Assignee

• PHARMAZELL GMBH · DE

Inventors

• Rolf Schmid · Gelterkinden, CH
• Michael Braun · Heidelberg, DE
• Luo Liu · Zhejiang, CN
• Arno Aigner · Bad Aibling, DE
• Dirk Weuster-Botz · Munich, DE

Key dates

• Filing date: May 27, 2011
• Grant date: Aug 4, 2015
• Expiry date: May 27, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y101/01159
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to 12 α-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 α-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 α-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12α-hydroxysteroids using said 12α-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 α-hydroxysteroid dehydrogenases.