Solid phase for capture of nucleic acids

US9096849B2 · kind B2 · utility

Assignee

• United States of America as represented by the Secretary of the Air Force · US

Inventors

• Marie J. Archer · Alexandria, US
• Baochuan Lin · Bethesda, US
• David A. Stenger · Reston, US

Key dates

• Filing date: May 21, 2007
• Grant date: Aug 4, 2015
• Expiry date: May 4, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07B2200/11
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.