Method of supplementing culture media to prevent undesirable amino acid substitutions
US9096879B2 · kind B2 · utility
17Cited by
8References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 24, 2009 |
| Grant date | Aug 4, 2015 |
| Priority date | — |
| Expiry date | Jan 7, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of reducing substitution of amino acids during the production of polypeptides of interest in mammalian cells. By varying the concentration of amino acids in the culture medium, heterogeneity of the polypeptide's amino acid sequence is decreased, leading to higher yields of functional protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.