Detection of bacteria and fungi
US9096884B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 19, 2010 |
| Grant date | Aug 4, 2015 |
| Priority date | — |
| Expiry date | Sep 8, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9015
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.