Sulfotransferase assay
US9096888B2 · kind B2 · utility
1Cited by
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11Claims
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Key dates
| Filing date | Jul 23, 2014 |
| Grant date | Aug 4, 2015 |
| Priority date | — |
| Expiry date | Jul 23, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2533/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of detecting sulfotransferase activity including conducting a first reaction comprising, measuring free phosphate produced by the first reaction, and comparing the measured free phosphate to a free phosphate standard curve or equation or to a free phosphate level obtained in a separate reaction. The first reaction includes combining the sulfotransferase, Golgi-resident PAP-phosphatase (gPAPP), a 5′-nucleotidase, a substrate of the sulfotransferase, and 3′-phosphoadenosine-5′-phosphosulfate (PAPS) under conditions to produce 3′-phosphoadenosine-5′-phosphate (PAP).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.