Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

US9102955B2 · kind B2 · utility

Assignee

• Lallemand Hungary Liquidity Management LLC · HU

Inventors

• John McBride · Lyme, US
• Elena Brevnova · Belmont, US
• Mark Mellon · Lebanon, US
• Allan Froehlich · Hartland, US
• Kristen M. Deleault · Canaan, US
• Vineet Rajgarhia · Dublin, US
• Riaan Den Haan · Durbanville, ZA
• Merja Penttila · Helsinki, FI
• Marja Ilmen · Helsinki, FI
• Matti Siika-Aho · Helsinki, FI
• Jaana Uusitalo · Espoo, FI
• Emily Stonehouse · Lebanon, US
• Alan Gilbert · Cambridge, US
• Haowen Xu · Lebanon, US
• Deidre Willes · Lebanon, US
• John S. Bardsley · Newport, US
• Anu Koivula · Kihniö, FI
• Sanni Voutilainen · Siuntio, FI

Key dates

• Filing date: Nov 23, 2009
• Grant date: Aug 11, 2015
• Expiry date: Nov 23, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.