Process for production of protein
US9102971B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 15, 2011 |
| Grant date | Aug 11, 2015 |
| Priority date | — |
| Expiry date | Dec 31, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.