Spider silk fusion protein structures for binding to an organic target

US9115204B2 · kind B2 · utility

Assignee

• SPIBER TECHNOLOGIES AB · SE

Inventors

• My Hedhammar · Stockholm, SE
• Jan Johansson · Stockholm, SE
• Anna Rising · Uppsala, SE
• Per Nygren · Helsingborg, SE

Key dates

• Filing date: Oct 25, 2011
• Grant date: Aug 25, 2015
• Expiry date: Jul 7, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S210/905
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.