Method for detecting gene modifications by means of asymmetrical PCR and blocking agents

US9115392B2 · kind B2 · utility

Assignee

• UNIVERSITÄT DUISBURG-ESSEN · DE

Inventors

• Martin Schuler · Bellinzona, CH
• Frank Breitenbuecher · Mülheim an der Ruhr, DE
• Sandra Hoffarth · Mülheim an der Ruhr, DE
• Sarah-Luise Stergar · Gelsenkirchen, DE

Key dates

• Filing date: Sep 9, 2010
• Grant date: Aug 25, 2015
• Expiry date: May 20, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6886
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of detecting at least one gene modification such as a mutation in a gene includes carrying out an asymmetric polymerase chain reaction (PCR) with a combined use of at least one detectable mutation-specific hybridization probe (sensor probe) and at least one wild-type specific blocking agent which inhibits a binding of the at least one detectable mutation-specific hybridization probe (sensor probe) to a wild-type gene so as to provide at least one of a selective intensification and an amplification of a detection of a gene segment of a mutation gene having a gene modification.