Detection of nucleic acids by target-specific hybrid capture method

US9115410B2 · kind B2 · utility

Assignee

• QIAGEN GAITHERSBURG INC. · US

Inventors

• Irina Nazarenko · Gaithersburg, US
• Attila Lorincz · Gaithersburg, US
• Paul Eder · Oreland, US
• Brian Lowe · Greer, US
• Richard Mallonee · Catonsville, US
• Ha Thai · Rockville, US

Key dates

• Filing date: Mar 15, 2013
• Grant date: Aug 25, 2015
• Expiry date: Jul 30, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6876
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.