Polymerase chain reaction product-cloning vector suitable to its easy production and method for producing the same
US9121023B2 · kind B2 · utility
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4Claims
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Key dates
| Filing date | Oct 29, 2008 |
| Grant date | Sep 1, 2015 |
| Priority date | — |
| Expiry date | Apr 28, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a PCR product cloning vector applicable directly to the cloning of a PCR product. More precisely, the present invention relates to a PCR product cloning vector designed to be effective in use for blue/white colony selection and produced based on the restriction enzyme treatment process to generate non-complementary unpaired single overhang and a method for producing the same. According to the method of the present invention, the PCR product cloning vector designed to be advantageous for blue/white cloning selection can be produced with improved efficiency compared with the convention method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.