Nanopore-based single molecule characterization
US9121059B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 21, 2011 |
| Grant date | Sep 1, 2015 |
| Priority date | — |
| Expiry date | Mar 8, 2034 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/924
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of using nanopore to obtain sequence information of an unknown structure (unknown DNA) in a ss test DNA. The method comprises using speed bump to stall the ss test DNA in the nanopore at random positions of the ss test DNA to obtain sequence information of each and every nucleotides of the unknown DNA, and to construct the whole sequence of the unknown DNA. The present invention also relates to a novel method of trapping a ss test DNA in a nanopore using two bulky structures formed under different conditions (e.g. different temperature), and the bulky structures are able to keep the ss test DNA trapped in a nanopore at a working temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.