Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
US9121061B2 · kind B2 · utility
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4References
24Claims
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Key dates
| Filing date | Mar 14, 2013 |
| Grant date | Sep 1, 2015 |
| Priority date | — |
| Expiry date | Mar 14, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/04005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.