Enzyme-based fed-batch technique in liquid cultures

US9127261B2 · kind B2 · utility

Assignee

• BioSilta Oy · FI

Inventors

• Peter Neubauer · Oulu, FI
• Antje Neubauer · Berlin, DE
• Antti Vasala · Oulu, FI

Key dates

• Filing date: Mar 4, 2010
• Grant date: Sep 8, 2015
• Expiry date: Mar 4, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is generally in the field of continuous and high-cell-density cultivation in laboratory- or large-scale liquid shaken cultures. More particularly it relates to a method of enzyme-based fed-batch (EnBase) for liquid microbial prokaryotic or eukaryotic cell cultivation having the possibility to manipulate the growth rate of the cultured organisms by a controlled enzymatic release of the growth-limiting substrate-monomer from substrate-polymers or substrate-oligomers.