Method for detection of beta-D-glucuronidase activity in a sample
US9127303B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 8, 2012 |
| Grant date | Sep 8, 2015 |
| Priority date | — |
| Expiry date | Jun 8, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detection of beta-D-glucuronidase activity in a sample that comprises the steps of (i) providing a glucuronic acid ester compound of the general formula (I) wherein R1 is a C1-4 alkyl group, OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond; (ii) contacting said glucuronic acid ester compound with a material of said sample exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.