Methods for performing direct enzymatic reactions involving nucleic acid molecules
US9127305B2 · kind B2 · utility
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12Claims
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Key dates
| Filing date | Feb 8, 2006 |
| Grant date | Sep 8, 2015 |
| Priority date | — |
| Expiry date | Jul 8, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for performing a direct enzymatic reaction involving a nucleic acid molecule include performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, in which a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction, and obtaining a product from the enzymatic reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.