O-phosphoserine sulfhydrylase mutants and method for production of cysteine using the same
US9127324B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 2011 |
| Grant date | Sep 8, 2015 |
| Priority date | — |
| Expiry date | Oct 20, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y205/01065
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.