Method of removing nucleic acid contamination in reverse transcription and amplification reactions

US9133447B2 · kind B2 · utility

Assignee

• Biotec Pharmacon Asa · NO

Inventors

• Olav Lanes · Tromsø, NO
• Morten Elde · Tromsø, NO
• Dag Rune Gjellesvik · Tromsø, NO

Key dates

• Filing date: Sep 25, 2013
• Grant date: Sep 15, 2015
• Expiry date: Dec 14, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.