High-speed maturation method for an oligonucleotide library for the purpose of preparing a protein library
US9133452B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 23, 2011 |
| Grant date | Sep 15, 2015 |
| Priority date | — |
| Expiry date | Jun 23, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of producing a maturated oligonucleotide library, including a step of obtaining a terminal-modified product of a maturation target oligonucleotide library, including adding a tag sequence to the 5′ terminus of the maturation target oligonucleotide library and an arrest sequence, which stalls translation elongation on a ribosome, to the 3′ terminus of the maturation target oligonucleotide library, a step of transcribing the terminal-modified sequence product to give a transcript, and a step of in vitro translation for translating the transcript in vitro, wherein the maturation target oligonucleotide library is a random oligonucleotide library.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.