Compositions and methods for diagnosis of genetic susceptibility, resistance, or tolerance to infection by mycobacteria and bovine paratuberculosis using promoter variants of EDN2

US9133519B2 · kind B2 · utility

Assignees

• WASHINGTON STATE UNIVERSITY · US
• UNIVERSITY OF MISSOURI · US
• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• Holly Neibergs · Palouse, US
• Ricardo Zanella · Caçapava do Sul, BR
• Jeremy Taylor · Columbia, US
• Zeping Wang · Pullman, US
• Erik Scraggs · Pullman, US
• Stephen N. White · Pullman, US
• Robert Schnabel · Columbia, US
• Curtis P. Van Tassell · Ellicott City, US

Key dates

• Filing date: Mar 15, 2013
• Grant date: Sep 15, 2015
• Expiry date: Dec 13, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are methods for determining susceptibility, resistance or tolerance to Mycobacterium avium subspecies paratuberculosis (Map) infection, comprising: determining, using a biological sample from a mammalian subject, a presence or absence of at least one EDN2 gene mutation, or the genotype of at least one EDN2 gene single nucleotide polymorphism (SNP) selected from the SNP group consisting of SNP105 (nucleotide position 105,288,174 on BTA3 (UMD 3.1)), SNP208 (nucleotide position 105,298,664 on BTA3 (UMD 3.1)), and SNP272 (nucleotide position 105,305,070 by on BTA3 (UMD 3.1)), that segregates with resistance and/or tolerance to Map tissue infection; and determining, based thereon, at least one of susceptibility, resistance or tolerance of the mammalian subject to Map infection. Additionally provided are mammals (e.g., bovine) derived culling, selecting, or selective breeding, based on disclosed methods, and methods for procuring mammals. Preferably, the mammals carry non-susceptible alleles of SNP105 (G), SNP208 (G), and SNP272 (G).