Systems and methods for using an immunostaining mask to selectively refine ISH analysis results
US9135694B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 4, 2012 |
| Grant date | Sep 15, 2015 |
| Priority date | — |
| Expiry date | Jan 15, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30072
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A computer-implemented method of processing image data representing biological units in a tissue sample includes receiving a first image of the tissue sample containing signals from an immunofluorescent (IF) morphological marker, wherein the tissue sample is stained with the IF morphological marker, and receiving a second image of the same tissue sample containing signals from a fluorescent probe, wherein the tissue sample is hybridized in situ with the fluorescent probe. The method further includes classifying each biological unit in the tissue sample into one of at least two classes based on a mean intensity of the signals from the IF morphological marker in the first image, performing a fluorescence in situ hybridization (FISH) analysis of the tissue sample in the second image to obtain results therefrom, and filtering the results of the FISH analysis to produce a subset of the results pertaining to biological units classified in one class.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.