Patent · US Active

Method for late introduction of the (8R)-hydroxyl group carbapenem β-lactam antibiotic synthesis

US9139588B2 · kind B2 · utility

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3References

3Claims

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Assignee

THE JOHNS HOPKINS UNIVERSITY · US

Inventors

Craig A. Townsend · Baltimore, US
Micah Bodner · Dexter, US
Ryan Martin Phelan · Baltimore, US
Michael Freeman · Bonn, DE

Key dates

Filing date	Dec 13, 2010
Grant date	Sep 22, 2015
Priority date	—
Expiry date	Dec 13, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P17/184
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The presently disclosed subject matter demonstrates that ThnG and ThnQ enzymes encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 giving N-acetyl thienamycin. ThnG catalyzes sequential desaturation and sulfoxidation of PS-5, giving PS-7 and its sulfoxide. The ThnG and ThnQ enzymes are relatively substrate selective, but give rise to the oxidative diversity of carbapenems produced by S. cattleya, and orthologues likely function similarly in allied streptomyces.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.