Fragment of humanized anti-EGFR antibody substituted-lysine variable fragment and use thereof
US9139650B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 2011 |
| Grant date | Sep 22, 2015 |
| Priority date | — |
| Expiry date | Jul 16, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/626
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The purpose of the present invention is to provide fragments of humanized anti-EGFR antibody substituted-lysine light-chain or heavy-chain variable regions, and single-chain antibodies, etc, comprising the same, having sufficient binding activity (affinity) with target cells, and having the ability to undergo various site-specific and uniform chemical modifications. The present invention pertains to a humanized variable region on the light-chain or heavy-chain of antibody 528 against human epidermal cell growth factor receptor 1 (Her-1), said variable region comprising the amino acid sequence represented by SEQ ID NO: 2 or SEQ ID NO: 4, wherein fragments of humanized anti-EGFR antibody lysine-substituted light-chain variable regions are formed by substituting a different amino acid for all of the lysine residues, or all of the lysine residues except for the lysine residue(s) of one specified moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.