Methods and compositions for targeting sequences of interest to the chloroplast

US9139842B2 · kind B2 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Henrik Albert · Alameda, US
• Linda A. Castle · Mountain View, US
• Matthew J. Heckert · Union City, US
• Jian-Ming Lu · Pearland, US
• Daniel L. Siehl · Menlo Park, US
• Yumin Tao · Ames, US

Key dates

• Filing date: Aug 12, 2011
• Grant date: Sep 22, 2015
• Expiry date: Mar 14, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y113/11027
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.