Assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity
US9140635B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 9, 2012 |
| Grant date | Sep 22, 2015 |
| Priority date | — |
| Expiry date | May 20, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B99/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.