Method of labeling a target nucleic acid
US9145586B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 2013 |
| Grant date | Sep 29, 2015 |
| Priority date | — |
| Expiry date | Sep 25, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of labeling and detecting a target nucleic acid by incubating a target nucleic acid with a terminal transferase to extend a terminus of the target nucleic acid and provide an extended region; hybridizing the extended region of the target nucleic acid with a template polynucleotide having a nucleotide sequence complementary to the extended region to obtain a hybridization product; and incubating the hybridization product with a nucleic acid polymerase and either a deoxynucleotide triphosphate (dNTP) having a detectable label or nucleotide triphosphate (NTP) having a detectable label to further extend the extended target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.