Patent · US Active

Chloroplast transit peptides and methods of their use

US9150625B2 · kind B2 · utility

0Cited by

3References

18Claims

0Family size

Assignee

E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

Timothy M. Bourett · Middletown, US
Gary C. Chun · Newark, US
Albert L. Lu · Newark, US

Key dates

Filing date	May 9, 2012
Grant date	Oct 6, 2015
Priority date	—
Expiry date	Aug 18, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/8221
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods and compositions are provided for targeting a polypeptide of interest to a chloroplast. Recombinant polynucleotides comprising a nucleotide sequence encoding a chimeric chloroplast transit peptide (CTP) operably linked to a heterologous polynucleotide of interest are provided. In specific embodiments, the chimeric CTP comprises an N-terminal domain operably linked to a central domain operably linked to a C-terminal domain of a CTP to form a chimeric chloroplast transit peptide having CTP activity. Recombinant polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the recombinant polynucleotides. Methods of use of the various sequences are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.