Self-complementary parvoviral vectors, and methods for making and using the same

US9150882B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Mark A. Kay · Los Altos, US
• Dirk Grimm · Palo Alto, US

Key dates

• Filing date: Jan 31, 2007
• Grant date: Oct 6, 2015
• Expiry date: Aug 16, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14152
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The teachings herein are generally directed to a method of enhancing the genetic stability of parvovirus vectors. The stability of conventional ss or dsAAV vector constructs can be enhanced, for example, to obtain a concurrent increase in vector titer and purity, as well as an improvement in vector safety, due at least in part to the elimination of stuffer DNA from the AAV vector. The method is broadly applicable to all gene transfer/therapy applications, such as those requiring delivery of foreign DNA containing recombinant gene expression cassettes. Such foreign DNA can range, for example, from about 0.2 up to about 5.2 kb in length. The enhanced vector constructs are highly flexible, user-friendly, and can be easily modified (via routine DNA cloning) and utilized (via standard AAV vector technology) by anyone skilled in the art.