Expression and purification of fusion protein with multiple MBP tags
US9150897B2 · kind B2 · utility
3Cited by
2References
19Claims
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Key dates
| Filing date | Aug 5, 2011 |
| Grant date | Oct 6, 2015 |
| Priority date | — |
| Expiry date | Aug 5, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BRCA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.