Oligonucleotides comprising a label associated through a linker

US9150912B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Daniel Müller · Inzlingen, DE
• Francesca Di Pasquale · Hilden, DE

Key dates

• Filing date: May 4, 2012
• Grant date: Oct 6, 2015
• Expiry date: May 4, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/107
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present application discloses a labeled nucleotide comprising a label attached via a linker, wherein said labeled nucleotide has the formula wherein R1 is a residue with a negative net charge, preferably selected from the group consisting of a phosphate group, and a sulphate group; wherein R2, R3 and R4 are independently selected from the group consisting of H2, OH2, and O; wherein “n” is an integer between 0 and 16; wherein “a” is an integer between 1 and 10; wherein SP is absent or a spacer; wherein X is said label; and wherein Y is a nucleotide or nucleoside. Furthermore, oligonucleotides comprising a labeled nucleotide according to the present invention and the use as a primer in amplification based methods is disclosed herein.