Nucleotide analogs

US9163053B2 · kind B2 · utility

Assignee

• FLUIDIGM CORPORATION · US

Inventors

• Suhaib Siddiqi · Raleigh, US
• J. William Efcavitch · San Mateo, US
• Judith Mitchell · Watertown, US
• Subramanian Marappan · Acton, US
• Jayson Bowers · Cambridge, US
• Mirna Jaroza · Arlington, US

Key dates

• Filing date: Dec 4, 2009
• Grant date: Oct 20, 2015
• Expiry date: Jul 15, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/107
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or ψ-uridine.