Patent · US Active

Method for culturing stem cells

US9163214B2 · kind B2 · utility

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12Claims

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Assignees

Inventors

Dongmei Lai · Shanghai, CN
Weiwei Cheng · Berlin, DE
Lihe Guo · Chenzhou, CN
Tianjin Liu · Novi, US
Lizhen Jiang · Shanghai, CN
Qin Huang · Wuxi, CN

Key dates

Filing date	May 4, 2010
Grant date	Oct 20, 2015
Priority date	—
Expiry date	Jul 27, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2502/025
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

In the field of biological technology, a stem cell culture method is provided. The method includes preparing an amniotic epithelial cell feeder layer that is not treated to lose the division ability; and seeding the stem cells onto the amniotic epithelial cell feeder layer, and culturing in a culture medium. The stem cell culture method according to the present invention does not require the treatment of the feeder layer cells to lose the division ability, and is thus simple and safe, thereby effectively solving the problem of contamination caused by animal-derived ingredients in culture of human stem cells at present, greatly reducing the culture cost of the stem cells, and providing a safe, effective, and inexpensive stem cell culture method for the industrialization of the stem cells in the future.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.