Patent · US Active

Method for isolating and purifying nucleic acids

US9163228B2 · kind B2 · utility

0Cited by

6References

21Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Roland Fabis · Leverkusen, DE
Markus Müller · Wegenstetten, CH
Jörg Hucklenbroich · Tente, DE
Mario Scherer · Hilden, DE

Key dates

Filing date	Apr 8, 2011
Grant date	Oct 20, 2015
Priority date	—
Expiry date	Oct 22, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/101
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.