Use of probes for unbound metabolites
US9164109B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 2007 |
| Grant date | Oct 20, 2015 |
| Priority date | — |
| Expiry date | Sep 11, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/728
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.