Production and extraction of procyanidins from plant cell cultures
US9167840B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 3, 2011 |
| Grant date | Oct 27, 2015 |
| Priority date | — |
| Expiry date | Feb 11, 2032 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H6/60
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of preparing cocoa oligomeric procyanidins from cocoa cell cultures grown in the presence of monosaccharide can increase production of procyanidins as follows: culturing cells sufficient to result in production of cocoa oligomeric procyanidins at a first rate; and introducing a monosaccharide to the cells sufficient for inducing the cells to produce the cocoa oligomeric procyanidins at a second rate that his higher than the first rate. The method can further include extracting the cocoa oligomeric procyanidins from the cells, and such extracting can occur between 1 day to 21 days after introduction of the monosaccharide. Optionally, the monosaccharide can be introduced in an amount about 0.5% to about to about 20% by volume of the culture medium. The monosaccharide can be glucose, sucrose, fructose, or the like. The monosaccharide can be introduced during or after a last phase of an exponential growth state.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.