Flooding dish and method for changing media in the dish in the preparation of mammalian specimen culture and for cryo-preservation, freezing, vitrification and the thawing and warming of such specimens
US9169460B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 19, 2009 |
| Grant date | Oct 27, 2015 |
| Priority date | — |
| Expiry date | Nov 16, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M23/12
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A tray assembly which may include a single well or a plurality of wells that will contain specimens for cryo-preservation, freezing, thawing, or warming. The assembly will include a structure whereby a sequence of different media solutions can be introduced into a well or wells in the assembly without the need to move the specimens and without disturbing an overlying oil layer which floats on top of the media solutions. The media solutions will be introduced into the specimen wells through adjunct passages which communicate with the interior of the wells. The adjunct passages will preferably be integral with the wells and will be formed at the same time the wells are formed. Thus manufacturing the assembly of this invention will be inexpensive and repetitive. The adjunct passages can be used to infuse different media solutions into the wells at different stages of the protocols in question. The wells can be inverted cones or pyramids in shape. This shape allows the infusion of sequential media solutions beneath the overlying oil layer, without disturbing the specimen and without significantly elevating the solution level in the wells. The conical or pyramidal shapes also enable the…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.