Process for the enzymatic production of C4 compounds from C6 substrates

US9169500B2 · kind B2 · utility

Inventors

• Volker Sieber · Dresden, DE
• André Pick · Bad Hersfeld, DE
• Broder Rühmann · Straubing, DE

Key dates

• Filing date: Mar 26, 2012
• Grant date: Oct 27, 2015
• Expiry date: Mar 26, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a novel process for converting a substrate of formula (III) and/or (IV) into a product of formula (I) or (II) comprising the following reactions: a) oxidation of at least one terminal C-atom, b) dehydratation, c) decarboxylation and d) reduction and/or amination. At least step b is enzyme-catalyzed. In a preferred embodiment, all reactions are enzymatically catalyzed. The enzymes catalyzing the reactions are selected from oxidoreductases, decarboxylases, dehydratases and/or aminotransferases. The process may be performed in a cell-free in vitro production system or in an improved fermentative production system.