High-sensitivity assays for pathogen detection using metal-enhanced fluorescence
US9170197B2 · kind B2 · utility
6Cited by
16References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 14, 2012 |
| Grant date | Oct 27, 2015 |
| Priority date | — |
| Expiry date | May 7, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/32
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an assay including a surface having silver colloids or islands attached thereto. Attached to the surface and/or silver colloids/islands are polynucleotides which are complimentary to a target polynucleotide sequence. The assay is performed by adding the target polynucleotide sequence to the assay surface and allowing it to hybridize with the capture polynucleotides. Fluorophore-labeled capture polynucleotides are added and hybridize to the target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.