Luciferin derivatives from bicyclic reactants and aminothiol derivatives and methods of use thereof

US9173966B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Christopher J. Chang · Berkeley, US
• Carolyn R. Bertozzi · Berkeley, US
• Genevieve Van de Bittner · Campbell, US
• Elena A. Dubikovskaya · Lausanne, CH

Key dates

• Filing date: Jan 15, 2013
• Grant date: Nov 3, 2015
• Expiry date: Jan 15, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/96466
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure features a condensation reaction and a luciferin-unmasking reaction that can be carried out under physiological conditions. In general, the condensation reaction involves reacting a bicyclic reactant with an aminothiol derivative, generating a luciferin or luciferin derivative. A luciferin can provide detectable luminescence. A luciferin derivative can be unmasked to provide detectable luminescence in a luciferin-unmasking reaction. The present disclosure provides bicyclic reactants and aminothiol derivatives suitable for use in the condensation reaction. The condensation and luciferin-unmasking reactions find use in a variety of applications, which are also provided.