Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition
US9175327B2 · kind B2 · utility
0Cited by
1References
12Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | May 3, 2011 |
| Grant date | Nov 3, 2015 |
| Priority date | — |
| Expiry date | Oct 6, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.