Methods for identifying nucleic acid modifications

US9175338B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Benjamin Flusberg · Atlanta, US
• Jonas Korlach · Camas, US
• Jeffrey Wegener · Cupertino, US
• Tyson A. Clark · Menlo Park, US
• Igor D. Vilfan · East Palo Alto, US
• Andrey Kislyuk · San Ramon, US
• Stephen Turner · Menlo Park, US
• Jon Sorenson · Alameda, US
• Kevin Travers · Menlo Park, US
• Cheryl Heiner · La Honda, US
• Austin B. Tomaney · Burlingame, US
• Patrick Marks · San Francisco, US
• Jessica Lee · Cupertino, US
• Lei Jia · Palo Alto, US
• Dale Webster · San Mateo, US
• John Lyle · Fremont, US
• Jeremiah Hanes · Woodside, US
• Joseph Puglisi · Stanford, US

Key dates

• Filing date: Nov 12, 2010
• Grant date: Nov 3, 2015
• Expiry date: Nov 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.