Methods for identifying nucleic acid modifications

US9175341B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Benjamin Flusberg · Atlanta, US
• Stephen Turner · Menlo Park, US
• Jessica Lee · Cupertino, US
• Lei Jia · Palo Alto, US
• Jonas Korlach · Camas, US
• Jon Sorenson · Alameda, US
• Dale Webster · San Mateo, US
• John Lyle · Fremont, US
• Kevin Travers · Menlo Park, US
• Jeremiah Hanes · Woodside, US
• Joseph Puglisi · Stanford, US

Key dates

• Filing date: Dec 10, 2009
• Grant date: Nov 3, 2015
• Expiry date: Jan 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2561/113
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.