System and method for carrying out multiple binding reactions in an array format

US9175421B2 · kind B2 · utility

Assignee

• BIO-RAD HAIFA LTD. · IL

Inventors

• Ariel G. Notcovich · Haifa, IL
• Alon Herschhorn · Nesher, IL
• Shai Nimri · Migdal HaEmek, IL
• John Barich · Pleasant Hill, US
• Ariel Lipson · Haifa, IL
• Ran Boaz · Haifa, IL
• Doron Lipson · Cambridge, US
• Yaakov Levie · Kiryat Shmona, IL

Key dates

• Filing date: Dec 21, 2011
• Grant date: Nov 3, 2015
• Expiry date: Jun 27, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00158
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.