Binding-induced hairpin detection system

US9176123B2 · kind B2 · utility

Assignee

• The Governors of the University of Alberta · CA

Inventors

• Xiaochun Le · Edmonton, CA
• Hongquan Zhang · Shanghai, CN
• Xing-Fang Li · Edmonton, CA

Key dates

• Filing date: Aug 30, 2010
• Grant date: Nov 3, 2015
• Expiry date: Jun 6, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.