Microscopic device and method for three-dimensional localization of point-like objects in a specimen
US9179131B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 9, 2012 |
| Grant date | Nov 3, 2015 |
| Priority date | — |
| Expiry date | Jan 30, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6421
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microscopic device for three-dimensional localization of point-like objects, encompassing a detection optical system that images point-like objects, each in the form of a three-dimensional focus light distribution, into an image space; a color separation apparatus that divides the light into at least two separate light bundles of different wavelength regions; at least two image space detector units , one receiving one light bundle and the other receiving the other light bundle, each detector unit comprising a light-spot-sensing detection surface ; an evaluation unit that ascertains a lateral X-Y position and an axial Z position relative to the sharpness plane in a direction perpendicular to the sharpness plane; at least one Z-position correction value for at least one of the wavelength regions being stored in the evaluation unit, which value indicates a detection optical system longitudinal chromatic aberration in that wavelength region; and the evaluation unit correcting the Z position.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.