Photodegradable groups for tunable polymeric materials

US9180196B2 · kind B2 · utility

Assignee

• The Regents of the University of Colorado, a Body Corporate · US

Inventors

• Kristi Anseth · Boulder, US
• Andrea Kasko · Boulder, US
• Mark W. Tibbitt · Boulder, US
• April M. Kloxin · Newark, US
• Balaji V. Sridhar · Denver, US

Key dates

• Filing date: Dec 21, 2012
• Grant date: Nov 10, 2015
• Expiry date: Mar 25, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G03F7/0037
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Here, we present a photodegradable microparticle system that can be employed to entrap and deliver bioactive proteins to cells during culture. By using a photosensitive delivery system, experimenters can achieve a wide variety of spatiotemporally regulated release profiles with a single microparticle formulation, thereby enabling one to probe many questions as to how protein presentation can be manipulated to regulate cell function. Photodegradable microparticles were synthesized via inverse suspension polymerization with a mean diameter of 22 μm, and degradation was demonstrated upon exposure to several irradiation conditions. The protein-loaded depots were incorporated into cell cultures and release of bioactive protein was quantified during the photodegradation process. This phototriggered release allowed for the delivery of TGF-β1 to stimulate PE25 cells and for the delivery of fluorescently labeled Annexin V to assay apoptotic 3T3 fibroblasts during culture. By incorporating these photoresponsive protein delivery depots into cell culture, new types of experiments are now possible to test hypotheses about how individual or multiple soluble factors might affect cell function whe…