Analysis of ubiquitinated polypeptides
US9181326B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 2013 |
| Grant date | Nov 10, 2015 |
| Priority date | — |
| Expiry date | Apr 4, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2440/36
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to antibody reagents that specifically bind to peptides carrying a ubiquitin remnant from a digested or chemically treated biological sample. The reagents allow the technician to identify ubiquitinated polypeptides as well as the sites of ubiquitination on them. The reagents are preferably employed in proteomic analysisusing mass spectrometry. The antibody reagents specifically bind to the remnant of ubiquitin (i.e., a diglycine modified epsilon amine of lysine) left on a peptide which as been generated by digesting or chemically treating ubiquitinated proteins. The inventive antibody reagents' affinity to the ubiquitin remnant does not depend on the remaining amino acid sequences flanking the modified (i.e., ubiquitinated) lysine, i.e., they are context independent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.